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peripheral blood lymphocyte separation medium  (Beyotime)


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    Beyotime peripheral blood lymphocyte separation medium
    Peripheral Blood Lymphocyte Separation Medium, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+peripheral+blood+lymphocyte+separation+medium/Human+Peripheral+Blood+Lymphocyte+Separation+Medium/pmc12915202-288-13-18
    Average 99 stars, based on 45 article reviews
    peripheral blood lymphocyte separation medium - by Bioz Stars, 2026-09
    99/100 stars

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    Isolation:

    Article Title: Differential expression of glycolysis-related genes and their potential immune mechanisms in acute myocardial infarction.
    Article Snippet: .. PBMCs were isolated by using Human Peripheral Blood Lymphocyte Separation Medium (C0025, Beyotime, China). .. Total RNA was extracted from the PBMCs by using Total RNA Extraction Kit (DP424, TIANGEN, China).

    Article Title: Differential expression of glycolysis-related genes and their potential immune mechanisms in acute myocardial infarction
    Article Snippet: .. PBMCs were isolated by using Human Peripheral Blood Lymphocyte Separation Medium (C0025, Beyotime, China). .. Total RNA was extracted from the PBMCs by using Total RNA Extraction Kit (DP424, TIANGEN, China).

    Article Title: Oxymatrine attenuates the type 1 diabetes mellitus via negative regulation of the follicular helper T cells.
    Article Snippet: Type 1 diabetes mellitus (T1DM) is an autoimmune disorder in which autoantibodies cause the immune system to attack and destroy pancreatic β-cells, leading to insufficient insulin production and impaired blood glucose control.. T follicular helper (Tfh) cells are recognized as a group of CD4 T cells that help B cells to produce highaffinity antibodies.. Our previous research found that oxymatrine (OMT) exhibits excellent immunomodulatory properties on Tfh cells in autoimmune diseases.

    Article Title: CD24 is a promising immunotherapeutic target for enhancing efficacy of third‐generation EGFR‐TKIs on EGFR‐mutated lung cancer
    Article Snippet: Phorbol 12‐myristate 13‐acetate (PMA) (100 μg/mL, Sigma‐Aldrich, St. Louis, MO, USA) was added to RPMI media to prime the THP‐1 monocytes into macrophage‐like cells (M0) for 48 h followed by washing the PMA off and a 24 h rest period in fresh media prior to exposure to polarizing cytokines. .. THP‐1 cells were differentiated into M2a and M2c with exposure to 20 ng/mL interleukin (IL)‐4 (Proteintech, Wuhan, Hubei, China) and IL‐13 (Proteintech) or IL‐10 (Proteintech) and transforming growth factor β1 (TGF‐β1) (Proteintech) for another 48 h. To generate M2 macrophages from human peripheral blood mononuclear cells (PBMCs), we first isolated PBMCs from whole blood samples donated by healthy volunteers using Human Peripheral Blood Lymphocyte Separation Medium (Beyotime). .. Next, we enrich CD14 positive monocytes from isolated PBMCs via Human CD14 MicroBeads UltraPure (Miltenyi Biotech, Cologne, Germany) according to the manufacturer's instructions.

    Flow Cytometry:

    Article Title: Identification of genetically engineered strategies to manipulate nano-platforms presenting immunotherapeutic ligands for alleviating primary ovarian insufficiency progression
    Article Snippet: .. Separation of peripheral blood mononuclear cells (PBMCs) was performed by Human Peripheral Blood Lymphocyte Separation Medium (Beyotime Biotechnology) and analyzed by the flow cytometry [ ]. .. Synthetic gene fragments encoding PD-L1 and Galectin-9 were obtained from Maipu Biotechnology (Nanjing, China) and subsequently subcloned into a PLV expression vector.

    Article Title: Identification of genetically engineered strategies to manipulate nano-platforms presenting immunotherapeutic ligands for alleviating primary ovarian insufficiency progression.
    Article Snippet: .. Separation of peripheral blood mononuclear cells (PBMCs) was performed by Human Peripheral Blood Lymphocyte Separation Medium (Beyotime Biotechnology) and analyzed by the flow cytometry [27]. .. Synthetic gene fragments encoding PD-L1 and Galectin-9 were obtained from Maipu Biotechnology (Nanjing, China) and subsequently subcloned into a PLV expression vector.

    Gradient Centrifugation:

    Article Title: Phosphoglycerate dehydrogenase interacts with and inhibits the protein kinase TAK1 to mitigate septic shock
    Article Snippet: .. Blood samples were collected by venipuncture, and PBMCs were separated by density gradient centrifugation using human peripheral blood lymphocyte separation medium (Cat# C0025, Beyotime) according to the manufacturer's instructions. ..



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